GCaMP3 is now officially old skool. GCaMP5 is available at addgene.
Plasmid 31788: pCMV-GCaMP5G, a.k.a. GCaMP3-T302L R303P D380Y
Dimmer baseline F, higher dF/F, picks up significantly more activity in vivo cortex. Still not holy grail level, but getting closer.
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How does it compare to the Campbell group’s GECOs?
Nobody knows, not even Rob. I believe his collaborators are trying to get data on action potential detection, but I’m not aware of any actual results on that front yet. The GECOs look potentially great for multicolor imaging, but until someone does in utero or virus injections with them there is no way to know.
Many, many sensors that look great in vitro fail miserably in vivo.
Great news. Any chance it’s available in an AAV plasmid (ie something akin to AAV-Syn-GCamP3 and ready to package)? Or better yet, are viral stocks available through UPenn or elsewhere? Thanks for the update.
Available via U-Penn:
V2340TI – AAV2/1.Syn.GCaMP5.WPRE.SV40 (p2401)
Cheers, Christian
Thanks!