A Better Neuronal Off-Switch

13 01 2010

Ed Boyden’s group has published High-performance genetically targetable optical neural silencing by light-driven proton pumps, detailing a set of new optical neuronal off-switches borrowed from various species that appear to be much better than Halorhodopsin for silencing neurons.  Halo works well for preventing action potentials when the nucleus is illuminated, but has a harder time blocking transmission of action potentials down an axon after it has been initiated. Also, previously engineered Halo variants, including eNpHR, suffer from light-dependent inactivation and have an expression sweet spot that could use broadening.  I’m looking forward to finding out if Arch, and the other new switches from Boyden’s group allow more powerful experiments in our hands.


Actions

Information

3 responses

13 01 2010
at

i’m surprised this made it to nature.

16 01 2010
Graham

The rule is “Nature (or Science) will not publish methods*”

* unless it is channelrhopdopsin-ish and thus will bump up the impact index.

26 03 2010
UPDATE : Bi-Directional Optogenetic Control « Brain Windows

[…] light intensities.  The paper implies superior performance over the Boyden group’s Arch optogenetic silencer technology, but shows no head to head data.  As always, testing both in your own system is the […]

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s




%d bloggers like this: