Journal Club : GFP Reconstitution Across Synaptic Partners (GRASP)

This week, I’m guest presenting a recent Neuron paper over at the Nature Network’s neuroscience journal club. The authors propose a clever new technique, Genetic Reconstitution Across Synaptic Partners, to track the locations of selected synaptic connections backed by an impressive set of in vivo proof-of-principal experiments in C. elegans. The gist of the strategy is to split a fluorescent marker into two non-functional components and then distribute each half on different sides of circuit’s connection. Only at synaptic connections would the two components be close enough to undergo trans-complementation and reconstitute a functional marker.

Check out the full presentation and join the discussion.